A new microphysiological system shows hypoxia primes human ISCs for interleukin-dependent rescue of stem cell activity.

Kristina R RiveraR Jarrett Bliton Joseph Burclaff Michael J CzerwinskiJintong LiuJessica M TruebloodCaroline M HinesleyKeith A BreauShlok JoshiVladimir A PozdinMing YaoAmanda L ZieglerAnthony T BlikslagerMichael A DanieleScott T Magness

Published in: bioRxiv : the preprint server for biology (2023)

Hypoxia primes hISCs to respond differently to interleukins than hISCs in normoxia through a transcriptional response. hISCs slow cell cycle progression and increase hISC activity when treated with hypoxia and specific interleukins. These findings have important implications for epithelial regeneration in the gut during inflammatory events.

Keyphrases

cell cycle
endothelial cells
stem cells
cell proliferation
gene expression
oxidative stress
mesenchymal stem cells
heat shock