Characterization of polypropyleneimine as an alternative transfection reagent.
Riku SaekiShingo KobayashiRena ShimazuiTeruki NiiAkihiro KishimuraTakeshi MoriMasaru TanakaYoshiki KatayamaPublished in: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2023)
Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.