Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production.
Evert K HolwerdaDaniel G OlsonNatalie M RuppertsbergerDavid M StevensonSean J L MurphyMarybeth I MaloneyAnthony A LanahanDaniel Amador-NoguezLee R LyndPublished in: Biotechnology for biofuels (2020)
Modifications in the organic acid fermentative pathways in Clostridium thermocellum caused an increase in extracellular pyruvate and free amino acids. Adaptive laboratory evolution led to improved growth, and an increase in ethanol yield and production due a mutation in adhE or a disruption in hfsB. Strains with deletions in ldh and pta pathways and subjected to ALE demonstrated undiminished cellulolytic capabilities when cultured on high cellulose loadings.