The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth.
Giulia L M BoezioShengnan ZhaoJosephine GollinRashmi PriyaShivani MansinghStefan GüntherNana FukudaFelix GunawanDidier Y R StainierPublished in: Disease models & mechanisms (2022)
The epicardium, the outermost layer of the heart, is an important regulator of cardiac regeneration. However, a detailed understanding of the crosstalk between the epicardium and myocardium during development requires further investigation. Here, we generated three models of epicardial impairment in zebrafish by mutating the transcription factor genes tcf21 and wt1a, and ablating tcf21+ epicardial cells. Notably, all three epicardial impairment models exhibited smaller ventricles. We identified the initial cause of this phenotype as defective cardiomyocyte growth, resulting in reduced cell surface and volume. This failure of cardiomyocyte growth was followed by decreased proliferation and increased abluminal extrusion. By temporally manipulating its ablation, we show that the epicardium is required to support cardiomyocyte growth mainly during early cardiac morphogenesis. By transcriptomic profiling of sorted epicardial cells, we identified reduced expression of FGF and VEGF ligand genes in tcf21-/- hearts, and pharmacological inhibition of these signaling pathways in wild type partially recapitulated the ventricular growth defects. Taken together, these data reveal distinct roles of the epicardium during cardiac morphogenesis and signaling pathways underlying epicardial-myocardial crosstalk.
Keyphrases
- left ventricular
- induced apoptosis
- signaling pathway
- transcription factor
- angiotensin ii
- heart failure
- stem cells
- genome wide
- single cell
- wild type
- endothelial cells
- cell surface
- dna methylation
- machine learning
- atrial fibrillation
- high glucose
- deep learning
- genome wide identification
- pi k akt
- vascular endothelial growth factor
- artificial intelligence
- catheter ablation