Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.
Ruoyu ZhongMatthew SullivanNeil UpretiRoy ChenAgustin De GanzóKaichun YangShujie YangKe JinYe HeKe LiJian-Ping XiaZhiteng MaLuke P LeeTania KonryTony Jun HuangPublished in: Science advances (2023)
The study of molecular mechanisms at the single-cell level holds immense potential for enhancing immunotherapy and understanding neuroinflammation and neurodegenerative diseases by identifying previously concealed pathways within a diverse range of paired cells. However, existing single-cell pairing platforms have limitations in low pairing efficiency, complex manual operation procedures, and single-use functionality. Here, we report a multiparametric cellular immunity analysis by a modular acoustofluidic platform: CIAMAP. This platform enables users to efficiently sort and collect effector-target (i.e., NK92-K562) cell pairs and monitor the real-time dynamics of immunological response formation. Furthermore, we conducted transcriptional and protein expression analyses to evaluate the pathways that mediate effector cytotoxicity toward target cells, as well as the synergistic effect of doxorubicin on the cellular immune response. Our CIAMAP can provide promising building blocks for high-throughput quantitative single-cell level coculture to understand intercellular communication while also empowering immunotherapy by precision analysis of immunological synapses.
Keyphrases
- single cell
- high throughput
- rna seq
- induced apoptosis
- immune response
- cell cycle arrest
- dendritic cells
- regulatory t cells
- gene expression
- signaling pathway
- traumatic brain injury
- endoplasmic reticulum stress
- stem cells
- oxidative stress
- cell death
- bone marrow
- transcription factor
- risk assessment
- type iii
- heat shock
- mass spectrometry
- lipopolysaccharide induced
- heat stress
- data analysis