Evaluation of different DNA extraction methods and loop-mediated isothermal amplification primers for the detection of Mycobacterium ulcerans in clinical specimens.

Anthony S AblordeyEvans AhotorCharles A NarhSandra A KingIsra CruzJoseph M Ndung'uDziedzom K de Souza

Published in: BMC infectious diseases (2021)

For further work on the development and use of LAMP tests for Buruli diagnosis, it is recommended that the BURULI sets of primers be used, as these yielded the best results in terms of sensitivity (87.50-91.49%) and specificity (89.23-100%), depending on the DNA extraction methods used.

Keyphrases

loop mediated isothermal amplification
circulating tumor
sensitive detection
cell free
single molecule
mycobacterium tuberculosis
circulating tumor cells
quantum dots
fine needle aspiration
ultrasound guided