Immunopeptidomics in the Era of Single-Cell Proteomics.
Rupert L MayerKarl MechtlerPublished in: Biology (2023)
Immunopeptidomics, as the analysis of antigen peptides being presented to the immune system via major histocompatibility complexes (MHC), is being seen as an imperative tool for identifying epitopes for vaccine development to treat cancer and viral and bacterial infections as well as parasites. The field has made tremendous strides over the last 25 years but currently still faces challenges in sensitivity and throughput for widespread applications in personalized medicine and large vaccine development studies. Cutting-edge technological advancements in sample preparation, liquid chromatography as well as mass spectrometry, and data analysis, however, are currently transforming the field. This perspective showcases how the advent of single-cell proteomics has accelerated this transformation of immunopeptidomics in recent years and will pave the way for even more sensitive and higher-throughput immunopeptidomics analyses.
Keyphrases
- mass spectrometry
- liquid chromatography
- single cell
- data analysis
- rna seq
- high resolution mass spectrometry
- capillary electrophoresis
- gas chromatography
- tandem mass spectrometry
- high performance liquid chromatography
- high resolution
- high throughput
- papillary thyroid
- sars cov
- squamous cell carcinoma
- young adults
- molecularly imprinted
- case control
- amino acid