MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata .
Lifu LiuXiaoming LiWei GuoJiajun ShiWenjun ChenYingying LeiYue MaHongyan DaiPublished in: Plants (Basel, Switzerland) (2022)
Alternaria alternata (A. alternata ) is a common pathogen that greatly influences apples' quantity and quality. However, chemical treatments produce increased health risks along with decreased food and environmental safety. Advancements in plant molecular biology, such as transgenic technology, have increased apple trees' resistance to pathogens and have therefore attracted widespread attention. WRKY transcription factors are involved in abiotic and biotic stress regulation; however, their biological role in non-model plants such as apple, is still unknown. In this investigation, MdWRKY120 was isolated from the 'GL-3' apple to determine its function during Alternaria alternate infection. The MdWRKY120-GFP fusion protein was located in the nucleus. MdWRKY120 in yeast cells exhibited activating transcriptional activity, meaning it is a transcription activator. MdWRKY120 overexpression transgenic plants were more sensitive to A. alternata , while RNAi transgenic plants showed increased resistance to A. alternata . This investigation demonstrates that MdWRKY120 enhances the susceptibility of apples to A. alternata .
Keyphrases
- transcription factor
- induced apoptosis
- signaling pathway
- genome wide identification
- cell proliferation
- gene expression
- working memory
- dna binding
- cell cycle arrest
- human health
- oxidative stress
- candida albicans
- cell death
- nuclear factor
- stress induced
- inflammatory response
- endoplasmic reticulum stress
- saccharomyces cerevisiae
- heat shock