Organic Anion Transporting Polypeptide 3A1 (OATP3A1)-Gated Bio-Orthogonal Labeling of Intracellular Proteins.
Krisztina NémethZsófia LászlóAdrienn BiróÁgnes SzatmáriGergely B CserépGyörgy VáradyÉva BakosCsilla Özvegy-LaczkaPéter KelePublished in: Molecules (Basel, Switzerland) (2023)
Organic anion transporting polypeptides (OATPs) were found to readily deliver membrane impermeable, tetrazine bearing fluorescent probes into cells. This feature was explored in OATP3A1 conditioned bio-orthogonal labeling schemes of various intracellular proteins in live cells. Confocal microscopy and super-resolution microscopy (STED) studies have shown that highly specific and efficient staining of the selected intracellular proteins can be achieved with the otherwise non-permeable probes when OATP3A1 is present in the cell membrane of cells. Such a transport protein linked bio-orthogonal labeling scheme is believed to be useful in OATP3A1 activity-controlled protein expression studies in the future.
Keyphrases
- induced apoptosis
- cell cycle arrest
- single molecule
- signaling pathway
- small molecule
- living cells
- endoplasmic reticulum stress
- machine learning
- high resolution
- cell death
- optical coherence tomography
- binding protein
- pi k akt
- high throughput
- fluorescence imaging
- protein protein
- deep learning
- single cell
- fluorescent probe
- high speed