Methylglyoxal disturbs DNA repair and glyoxalase I system in Saccharomyces cerevisiae.
Sandra Sartoretto PavinAlessandro de Souza PrestesMatheus Mulling Dos SantosGabriel Teixeira de MacedoSabrina Antunes FerreiraMariana Torri ClaroCristiane Lenz Dalla CorteNilda Vargas BarbosaPublished in: Toxicology mechanisms and methods (2020)
Methylglyoxal (MG) is a highly reactive aldehyde able to form covalent adducts with proteins and nucleic acids, disrupting cellular functions. In this study, we performed a screening of Saccharomyces cerevisiae (S. cerevisiae) strains to find out which genes of cells are responsive to MG, emphasizing genes against oxidative stress and DNA repair. Yeast strains were grown in the YPD-Galactose medium containing MG (0.5 to 12 mM). The tolerance to MG was evaluated by determining cellular growth and cell viability. The toxicity of MG was more pronounced in the strains with deletion in genes engaged with DNA repair checkpoint proteins, namely Rad23 and Rad50. MG also impaired the growth and viability of S. cerevisiae mutant strains Glo1 and Gsh1, both components of the glyoxalase I system. Differently, the strains with deletion in genes encoding for antioxidant enzymes were apparently resistant to MG. In summary, our data indicate that DNA repair and MG detoxification pathways are keys in the control of MG toxicity in S. cerevisiae.
Keyphrases
- dna repair
- dna damage
- saccharomyces cerevisiae
- oxidative stress
- dna damage response
- escherichia coli
- induced apoptosis
- genome wide
- machine learning
- cell death
- dna methylation
- genome wide identification
- cell cycle
- signaling pathway
- endoplasmic reticulum stress
- cell proliferation
- deep learning
- big data
- electronic health record
- data analysis
- diabetic rats
- ischemia reperfusion injury