Electrospray ionization and heterogeneous matrix effects in liquid chromatography/mass spectrometry based meta-metabolomics: A biomarker or a suppressed ion?
Hikmat GhossonYann GuittonAmani Ben JradChandrashekhar D PatilDelphine RaviglioneMarie-Virginie SalviaCédric BertrandPublished in: Rapid communications in mass spectrometry : RCM (2021)
Ion suppression should be considered as a critical issue for biomarker determination when comparing heterogeneous metabolic profiles. Raw chromatograms and mass spectra crosschecks are mandatory to reveal potential ion suppressions in such cases. Dilution is a suitable approach to filter reliable biomarker candidates before their identification and absolute quantification.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution mass spectrometry
- gas chromatography
- tandem mass spectrometry
- solid phase extraction
- high performance liquid chromatography
- capillary electrophoresis
- genome wide
- dna methylation
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- risk assessment
- density functional theory
- gene expression
- human health