In Situ Biosynthesized Superparamagnetic Iron Oxide Nanoparticles (SPIONS) Induce Efficient Hyperthermia in Cancer Cells.
Swati KaushikJijo ThomasVineeta PanwarHasan AliVianni ChopraAnjana SharmaRuchi TomarDeepa GhoshPublished in: ACS applied bio materials (2020)
Despite the promising role of magnetic hyperthermia in cancer therapy, its use in patients has been restricted by hurdles that include inefficient targeting of magnetic particles to the tumor site, limited bioavailability, and high toxicity, etc. Taking advantage of the unique metabolic property of cancer cells, we explored the potential of these cells to biosynthesize magnetic nanoparticles for potential hyperthermia applications. Treatment of cancer cells with a mixture of FeCl 2 and zinc gluconate resulted in a significant increase in intracellular Fe and Zn content in these cells. Exposure of these cells to an alternating magnetic field (AMF) for 30 min resulted in a substantial temperature rise of 5-6 °C. The in situ formed particles were identified as iron oxide and ZnO nanoparticles. Based on the magnetic property and size, the iron oxide nanoparticles were classified as superparamagnetic iron oxide nanoparticles (SPIONS) comprising a mixture of magnetite (Fe 3 -δO 4 ) and maghemite (γ-Fe 2 O 3 ). The role of reactive oxygen species (H 2 O 2 ) and the involvement of the glycolytic pathway in the biosynthesis of the nanoparticles were confirmed using appropriate in vitro studies. The simplicity of treatment, the specificity of cells capable of synthesis of SPIONS, and the hyperthermia response observed in cancer cells indicate a promising strategy to achieve effective magnetic hyperthermia for cancer therapy.
Keyphrases
- iron oxide nanoparticles
- induced apoptosis
- cancer therapy
- cell cycle arrest
- reactive oxygen species
- oxidative stress
- iron oxide
- molecularly imprinted
- endoplasmic reticulum stress
- magnetic nanoparticles
- chronic kidney disease
- end stage renal disease
- risk assessment
- newly diagnosed
- cell proliferation
- mass spectrometry
- signaling pathway
- high resolution
- smoking cessation
- peritoneal dialysis
- replacement therapy
- liquid chromatography
- walled carbon nanotubes