Glucosinolates of Lepidium graminifolium L. (Brassicaceae) from Croatia.

The glucosinolate (GSL) profiles (inflorescence, stem, root, and fruit) of the wild-growing plant Lepidium graminifolium L. (Brassicaceae) from Croatia was established by LC-MS analysis. During this investigation, we confirmed the presence of benzyl- (1), 3-methoxybenzyl- (2), 4-hydroxybenzyl- (4), 4-methoxyindol-3-ylmethyl- (7) GSLs and reported for the first time in the plant the presence of (2 R)-hydroxybut-3-enyl- (11), (2S)-hydroxybut-3-enyl- (12), but-3-enyl- (13), and 2-phenylethyl- (14) GSLs. Finally, 3-hydroxybenzyl GSL (3) was isolated for the first time from L. graminifolium inflorescence and characterised by spectroscopic data interpretation.