cAMP Biosensors Based on Genetically Encoded Fluorescent/Luminescent Proteins.
Namdoo KimSeunghan ShinSe Won BaePublished in: Biosensors (2021)
Cyclic adenosine monophosphate (cAMP) plays a key role in signal transduction pathways as a second messenger. Studies on the cAMP dynamics provided useful scientific insights for drug development and treatment of cAMP-related diseases such as some cancers and prefrontal cortex disorders. For example, modulation of cAMP-mediated intracellular signaling pathways by anti-tumor drugs could reduce tumor growth. However, most early stage tools used for measuring the cAMP level in living organisms require cell disruption, which is not appropriate for live cell imaging or animal imaging. Thus, in the last decades, tools were developed for real-time monitoring of cAMP distribution or signaling dynamics in a non-invasive manner. Genetically-encoded sensors based on fluorescent proteins and luciferases could be powerful tools to overcome these drawbacks. In this review, we discuss the recent genetically-encoded cAMP sensors advances, based on single fluorescent protein (FP), Föster resonance energy transfer (FRET), single luciferase, and bioluminescence resonance energy transfer (BRET) for real-time non-invasive imaging.
Keyphrases
- energy transfer
- quantum dots
- binding protein
- protein kinase
- early stage
- high resolution
- living cells
- signaling pathway
- radiation therapy
- mass spectrometry
- mesenchymal stem cells
- cell proliferation
- epithelial mesenchymal transition
- small molecule
- induced apoptosis
- multidrug resistant
- rectal cancer
- endoplasmic reticulum stress
- fluorescence imaging
- gram negative
- fluorescent probe
- case control
- locally advanced