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Performance comparison of two commercial human whole-exome capture systems on formalin-fixed paraffin-embedded lung adenocarcinoma samples.

Silvia BonfiglioIrene VanniValeria RossellaAnna TruiniDejan LazarevicMaria Giovanna Dal BelloAngela AlamaMarco MoraErika RijavecCarlo GenovaDavide CittaroFrancesco GrossiSimona Coco
Published in: BMC cancer (2016)
High-quality and reliable data can be successfully obtained from WES of FFPE samples starting from a relatively low amount of input gDNA, suggesting the inclusion of NGS-based tests into clinical contest. In conclusion, our analysis suggests that the WES approach could be extended to a translational research context as well as to the clinic (e.g. to study rare malignancies), where the simultaneous analysis of the whole coding region of the genome may help in the detection of cancer-linked variants.
Keyphrases
  • copy number
  • endothelial cells
  • papillary thyroid
  • primary care
  • induced pluripotent stem cells
  • loop mediated isothermal amplification
  • label free
  • machine learning
  • sensitive detection