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Age Trends in Growth and Differentiation Factor-11 and Myostatin Levels in Healthy Men, Measured Using Liquid Chromatography Tandem Mass Spectrometry: Differential Response to Testosterone.

Liming PengThiago Gagliano-JucáKarol M PencinaSrinivasan KrishnanZhuoying LiRussell P TracyRavi JasujaShalender Bhasin
Published in: The journals of gerontology. Series A, Biological sciences and medical sciences (2021)
The LC-MS/MS method for the simultaneous measurement of circulating total GDF-8 and GDF-11 demonstrates the characteristics of a valid assay. Testosterone treatment increased GDF-8 levels, but not GDF-11. Increase in GDF-8 levels by testosterone treatment which increased muscle mass, suggests that GDF-8 acts as a chalone to restrain muscle growth.
Keyphrases
  • liquid chromatography tandem mass spectrometry
  • replacement therapy
  • simultaneous determination
  • skeletal muscle
  • ms ms
  • solid phase extraction
  • middle aged