A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM).

Wei-Hsuan LeeJian-Zong LaiYu-Hsuan HsuFung-Yu ChengChing-Lung LuoYung-Chin HuangTzu-Chau Lin Fan-Ching Chien

Published in: Chemical communications (Cambridge, England) (2021)

A small-sized chromophore, BTTA-2OH, manifesting favorable solubility, large two-photon excitation efficiency, and good fluorescence photostability was synthesized to label the membrane of living cells for visualizing the dynamic movement of membrane-related vesicles via a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy.

Keyphrases

living cells
single molecule
energy transfer
fluorescence imaging
high resolution
fluorescent probe
photodynamic therapy
quantum dots
high throughput
mass spectrometry
optical coherence tomography
high speed