The effects of short term hyperglycemia on human red blood cells studied using Raman spectroscopy and optical trap.
Yashveer SinghAniket ChowdhuryRaktim DasguptaShovan Kumar MajumderPublished in: European biophysics journal : EBJ (2021)
Management of postprandial hyperglycemia is important for preventing severe complications like cardiovascular disease in diabetes patients. The associated glycemic instability in postprandial hyperglycemia may also cause disorders in circulating red blood cells (RBCs). Therefore, effects of short-term hyperglycemic stress on RBCs such as occur in the postprandial condition, have been studied here ex vivo using single-cell Raman spectroscopy and optical trapping. RBCs incubated in high glucose containing media relevant to postprandial hyperglycemia were studied for changes with respect to controls by analyzing the single-cell Raman spectra acquired with Raman optical tweezers with 532 nm excitation light. Use of 532 nm light for exciting Raman spectra also results in simultaneous photoreduction of intracellular hemoglobin (Hb). The level of photoreduction was noticed to be limited in hyperglycemia-exposed cells in comparison to the control. Since this suggests formation of permanently oxidized Hb in hyperglycemia-exposed RBCs, a fluorescence study was performed which showed elevated levels of oxidative stress in these cells. The changes in the RBC membrane, which may result due to higher levels of oxidative stress, were investigated using optical stretching experiments under the laser trap. The results indicated a loss of elasticity for the RBC membrane due to hyperglycemic exposure.
Keyphrases
- raman spectroscopy
- red blood cell
- diabetic rats
- oxidative stress
- induced apoptosis
- single cell
- cardiovascular disease
- high speed
- endothelial cells
- high resolution
- type diabetes
- blood glucose
- high glucose
- cell cycle arrest
- rna seq
- endoplasmic reticulum stress
- end stage renal disease
- dna damage
- chronic kidney disease
- density functional theory
- atomic force microscopy
- high throughput
- early onset
- metabolic syndrome
- cardiovascular events
- adipose tissue
- blood pressure
- drug induced
- cell proliferation
- weight loss
- heat stress
- low density lipoprotein