Arabidopsis OXIDATIVE STRESS 3 enhances stress tolerance in Schizosaccharomyces pombe by promoting histone subunit replacement that upregulates drug-resistant genes.
Dingwang LaiXiuting HuangChanghu WangDavid W OwPublished in: Genetics (2022)
Histone replacement in chromatin-remodeling plays an important role in eukaryotic gene expression. New histone variants replacing their canonical counterparts often lead to a change in transcription, including responses to stresses caused by temperature, drought, salinity, and heavy metals. In this study, we describe a chromatin-remodeling process triggered by eviction of Rad3/Tel1-phosphorylated H2Aα, in which a heterologous plant protein AtOXS3 can subsequently bind fission yeast HA2.Z and Swc2, a component of the SWR1 complex, to facilitate replacement of H2Aα with H2A.Z. The histone replacement increases occupancy of the oxidative stress-responsive transcription factor Pap1 at the promoters of at least three drug-resistant genes, which enhances their transcription and hence primes the cell for higher stress tolerance.
Keyphrases
- drug resistant
- transcription factor
- dna methylation
- gene expression
- genome wide
- oxidative stress
- multidrug resistant
- dna damage
- genome wide identification
- acinetobacter baumannii
- heavy metals
- copy number
- dna binding
- ischemia reperfusion injury
- single cell
- heat stress
- microbial community
- cancer therapy
- saccharomyces cerevisiae
- diabetic rats
- climate change
- pseudomonas aeruginosa
- cell wall
- heat shock
- stress induced
- health risk
- induced apoptosis
- health risk assessment
- protein kinase