VITAS, a sensitive in vivo selection assay to discover enzymes producing antiviral natural products.

Aws Fahd AlharbiHayun KimDhirish ChumrooYuxuan JiMohammed HakilKourosh Honarmand Ebrahimi

Published in: Chemical communications (Cambridge, England) (2023)

To discover new broad-spectrum antiviral nucleotide analogues from natural resources or through protein engineering, we have developed a sensitive in vivo selection assay named Viral polymerase-Inhibition Toxin-Associated Selection (VITAS). We show that the assay works with enzymes from three Kingdoms of life.

Keyphrases

high throughput
escherichia coli
sars cov
molecular docking
protein protein
small molecule
single cell
molecular dynamics simulations