Reusable Electronic Tongue Based on TRPV1 Nanodisc-conjugated Graphene Field-effect Transistor for a Spiciness-related Pain Evaluation.

The sense of spiciness is related to the stimulation of vanilloid compounds contained in the foods. Although, the spiciness is commonly considered as the part of taste, it is more classified to the sense of pain stimulated on a tongue, namely, pungency, which is described as a tingling or burning on the tongue. Herein, w e firstly fabricated a reusable electronic tongue based on a TRPV1 nanodisc conjugated graphene field-effect transistor and demonstrated spiciness-related pain evaluation with reusable electrode. The pungent compound reactive receptor TRPV1 w as synthesized in the form of nanodiscs to maintain stability and reusability. O ur newly developed platform showed highly selective and sensitive performance towards each spiciness related vanilloid compound repeatably: 1 aM capsaicin, 10 aM dihydrocapsaicin, 1 fM piperine, 10 nM allicin, and 1 pM AITC. The binding mechanism w as also examined by simulation. Furthermore, the elimination of the burning sensation on the tongue after eating spicy foods w as not investigated. Based on the synthesis of micelles composed of casein protein (which is contained in skim milk) that remove pungent compounds bound to TRPV1 nanodisc, the deactivation of TRPV1 w as investigated, and the electrode w as reusable that mimicking electronic tongue. This article is protected by copyright. All rights reserved.