Molecular Epidemiology of blaKPC-Encoding Klebsiella pneumoniae Isolated from Public Hospitals in Midwest of Brazil.
Ana Claudia Souza RodriguesMarilene Rodrigues ChangIvson Cassiano de Oliveira SantosAna Paula D' Alincourt Carvalho AssefPublished in: Microbial drug resistance (Larchmont, N.Y.) (2021)
This study was conducted to determine the molecular epidemiology of blaKPC-encoding Klebsiella pneumoniae recovered from three public hospitals in Brazil. Molecular investigation of blaOXA-48, blaKPC, blaNDM, blaCTX-M, blaSHV, blaTEM, blaIMP, and blaVIM resistance genes was performed in 99 K. pneumoniae isolates from inpatients of intensive care units. Antimicrobial susceptibility was determined with a Vitek-2 System, except for polymyxin B, which was evaluated by the microbroth dilution test. Clonal relatedness was established by pulsed-field gel electrophoresis and multilocus sequence typing. Screening resistance genes showed that K. pneumoniae isolates carried the blaKPC (88.9%), blaSHV (73.5%), blaTEM (72.2%), and blaCTX-M (43.9%) genes. The most frequent sequence types (STs) were ST273, ST11, ST 1298, ST13, ST2687, and ST37. We report new STs in K. pneumoniae that have not been detected previously in Brazil. K. pneumoniae belonging to the same clone is present in different hospitals in the same region, showing the spread of multidrug-resistant K. pneumoniae.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- escherichia coli
- healthcare
- gram negative
- drug resistant
- acinetobacter baumannii
- genome wide
- intensive care unit
- respiratory tract
- mental health
- genome wide identification
- amino acid
- pseudomonas aeruginosa
- ms ms
- dna methylation
- mass spectrometry
- adverse drug
- single molecule
- solid phase extraction