Integrating optical tweezers with up-converting luminescence: a non-amplification analytical platform for quantitative detection of microRNA-21 sequences.

Cheng-Yu LiDi CaoChong-Yang SongChun-Miao XuXu-Yan MaZhi-Ling ZhangDai-Wen PangHong-Wu Tang

Published in: Chemical communications (Cambridge, England) (2018)

We report a single-microsphere based imaging assay method by integrating up-converting luminescence with optical tweezers for detecting microRNA-21 sequences. This method achieves a competitive detection limit of 12 fM with good selectivity and no dedicated signal amplification designs.

Keyphrases

high resolution
label free
high throughput
loop mediated isothermal amplification
quantum dots
nucleic acid
real time pcr
high speed
energy transfer
mass spectrometry
structural basis