Vibrational Spectroscopy for Identification of Metabolites in Biologic Samples.
Kevin V HackshawJoseph S MillerDidem Peren AykasLuis Rodriguez-SaonaPublished in: Molecules (Basel, Switzerland) (2020)
Vibrational spectroscopy (mid-infrared (IR) and Raman) and its fingerprinting capabilities offer rapid, high-throughput, and non-destructive analysis of a wide range of sample types producing a characteristic chemical "fingerprint" with a unique signature profile. Nuclear magnetic resonance (NMR) spectroscopy and an array of mass spectrometry (MS) techniques provide selectivity and specificity for screening metabolites, but demand costly instrumentation, complex sample pretreatment, are labor-intensive, require well-trained technicians to operate the instrumentation, and are less amenable for implementation in clinics. The potential for vibration spectroscopy techniques to be brought to the bedside gives hope for huge cost savings and potential revolutionary advances in diagnostics in the clinic. We discuss the utilization of current vibrational spectroscopy methodologies on biologic samples as an avenue towards rapid cost saving diagnostics.
Keyphrases
- high resolution
- mass spectrometry
- high throughput
- single molecule
- magnetic resonance
- primary care
- ms ms
- density functional theory
- rheumatoid arthritis
- molecular dynamics simulations
- solid state
- raman spectroscopy
- energy transfer
- liquid chromatography
- multiple sclerosis
- healthcare
- magnetic resonance imaging
- climate change
- resistance training
- quality improvement
- high frequency
- gas chromatography
- body composition
- high intensity
- bioinformatics analysis
- structural basis