A Library of Potential Nanoparticle Contrast Agents for X-Ray Fluorescence Tomography Bioimaging.
Yuyang LiKian ShakerJakob C LarssonCarmen VogtHans M HertzMuhammet Sadaka ToprakPublished in: Contrast media & molecular imaging (2018)
Nanoparticles (NPs) have been used as contrast agents for several bioimaging modalities. X-ray fluorescence (XRF) tomography can provide sensitive and quantitative 3D detection of NPs. With spectrally matched NPs as contrast agents, we demonstrated earlier in a laboratory system that XRF tomography could achieve high-spatial-resolution tumor imaging in mice. Here, we present the synthesis, characterization, and evaluation of a library of NPs containing Y, Zr, Nb, Rh, and Ru that have spectrally matched K-shell absorption for the laboratory scale X-ray source. The K-shell emissions of these NPs are spectrally well separated from the X-ray probe and the Compton background, making them suitable for the lab-scale XRF tomography system. Their potential as XRF contrast agents is demonstrated successfully in a small-animal equivalent phantom, confirming the simulation results. The diversity in the NP composition provides a flexible platform for a better design and biological optimization of XRF tomography nanoprobes.
Keyphrases
- electron microscopy
- high resolution
- dual energy
- magnetic resonance
- quantum dots
- single molecule
- contrast enhanced
- oxide nanoparticles
- living cells
- computed tomography
- energy transfer
- fluorescent probe
- magnetic resonance imaging
- fluorescence imaging
- risk assessment
- pet imaging
- sensitive detection
- label free
- real time pcr
- virtual reality
- solid state