Angiopteris cochinchinensis de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition.
Won Young JangHwa Pyoung LeeSeung A KimLei HuangJi Hye YoonChae Yun ShinAnkita MitraHan Gyung KimYoung-Jin SonPublished in: Plants (Basel, Switzerland) (2022)
Growing demand for treatment options against acute lung injury (ALI) emphasizes studies on plant extracts harboring anti-inflammatory effects. According to GC-MS analysis, Angiopteris cochinchinensis de Vriese consists of various flavonoids with anti-inflammatory activities. Thus, in this study, the anti-inflammatory effects of an extract of Angiopteris cochinchinensis de Vriese (Ac-EE) were assessed using RAW264.6 murine macrophages and a lipopolysaccharide (LPS)-induced ALI model. Ac-EE reduced the nitric oxide production in murine macrophages increased by LPS induction. Moreover, protective effects of Ac-EE on lung tissue were demonstrated by shrinkage of edema and lung injury. Reduced neutrophil infiltration and formation of hyaline membranes were also detected in lung tissues after H&E staining. Semiquantitative RT-PCR, quantitative real-time PCR, and ELISA showed that Ac-EE inhibits the production of proinflammatory mediators, including i NOS and COX-2, and cytokines, such as TNF-α, IL-1β, and IL-6. An Ac-EE-mediated anti-inflammatory response was derived from inhibiting the NF-κB signaling pathway, which was evaluated by luciferase reporter assay and Western blotting analysis. A cellular thermal shift assay revealed that the prime target of Ac-EE in alleviating inflammation was Src. With its direct binding with Src, Angiopteris cochinchinensis de Vriese significantly mitigates lung injury, showing possibilities of its potential as an effective botanical drug.
Keyphrases
- lps induced
- inflammatory response
- anti inflammatory
- lipopolysaccharide induced
- signaling pathway
- toll like receptor
- nitric oxide
- real time pcr
- tyrosine kinase
- oxidative stress
- rheumatoid arthritis
- gene expression
- high throughput
- radiation therapy
- pi k akt
- south africa
- single cell
- mouse model
- crispr cas
- high resolution
- hydrogen peroxide
- cell proliferation
- radiation induced
- adverse drug
- electronic health record
- monoclonal antibody
- binding protein