Production of a recombinant swollenin from Trichoderma harzianum in Escherichia coli and its potential synergistic role in biomass degradation.
Clelton A SantosJaire A Ferreira-FilhoAnthonia O'DonovanVijai K GuptaMaria G TuohyAnete Pereira de SouzaPublished in: Microbial cell factories (2017)
Many aspects of the structure and mechanism of action of fungal SWOs remain unknown. In the present study, we produced a recombinant, active SWO from T. harzianum using a prokaryotic host and confirmed its potential synergistic role in biomass degradation. Our work paves the way for further studies evaluating the structure and function of this protein, especially regarding its use in biotechnology.