Inactivation of common airborne antigens by perfluoroalkyl chemicals modulates early life allergic asthma.
Mengjing WangQianqian LiMeifang HouLouisa L Y ChanMeng LiuSoo Kai TerTing DongYun XiaSanjay Haresh ChotirmallMingliang FangPublished in: Proceedings of the National Academy of Sciences of the United States of America (2021)
Allergic asthma, driven by T helper 2 cell-mediated immune responses to common environmental antigens, remains the most common respiratory disease in children. Perfluorinated chemicals (PFCs) are environmental contaminants of great concern, because of their wide application, persistence in the environment, and bioaccumulation. PFCs associate with immunological disorders including asthma and attenuate immune responses to vaccines. The influence of PFCs on the immunological response to allergens during childhood is unknown. We report here that a major PFC, perfluorooctane sulfonate (PFOS), inactivates house dust mite (HDM) to dampen 5-wk-old, early weaned mice from developing HDM-induced allergic asthma. PFOS further attenuates the asthma protective effect of the microbial product lipopolysaccharide (LPS). We demonstrate that PFOS prevents desensitization of lung epithelia by LPS, thus abolishing the latter's protective effect. A close mechanistic study reveals that PFOS specifically binds the major HDM allergen Der p1 with high affinity as well as the lipid A moiety of LPS, leading to the inactivation of both antigens. Moreover, PFOS at physiological human (nanomolar) concentrations inactivates Der p1 from HDM and LPS in vitro, although higher doses did not cause further inactivation because of possible formation of PFOS aggregates. This PFOS-induced neutralization of LPS has been further validated in primary human cell models and extended to an in vivo bacterial infection mouse model. This study demonstrates that early life exposure of mice to a PFC blunts airway antigen bioactivity to modulate pulmonary inflammatory responses, which may adversely affect early pulmonary health.
Keyphrases
- allergic rhinitis
- early life
- inflammatory response
- chronic obstructive pulmonary disease
- lung function
- immune response
- dendritic cells
- endothelial cells
- anti inflammatory
- mouse model
- high glucose
- human health
- single cell
- pulmonary hypertension
- healthcare
- cell therapy
- microbial community
- induced pluripotent stem cells
- mental health
- oxidative stress
- high fat diet induced
- drug induced
- drinking water
- mesenchymal stem cells
- health risk assessment
- metabolic syndrome
- stem cells
- bone marrow
- health promotion
- insulin resistance