Development, validation and application of a gas chromatography method for the determination of dillapiole from Piper aduncum essential oil in skin permeation samples.
Simone Braga CarneiroTainá KreutzRenata Pereira LimbergerValdir Florêncio da Veiga JúniorLetícia Scherer KoesterPublished in: Biomedical chromatography : BMC (2022)
The essential oil extracted from the leaves of Piper aduncum has antifungal, insecticidal and antibacterial activity. Studies with its main compound, dillapiole (DIL) revealed antibacterial and anti-inflammatory potential. Despite all this bioactivity, there is no updated report on the development and validation of analytical and bioanalytical methodology to quantify DIL in skin samples. A selective, precise, accurate and adequate method for the determination of DIL in solutions, porcine ear skin samples and receptor fluid was developed and validated by headspace extraction-gas chromatography with flame ionization detection (HS-GC-FID). HS-GC-FID was applied to determine DIL in Franz cell permeation and retention studies using porcine ear skin samples. In the HS-GC-FID method, matrix-related interferences were not observed at the peak of the DIL retention time. The results showed a high recovery (>97%) after the extraction procedure, allowing the quantification of DIL in complex matrices. In vitro permeation/retention for DIL showed cumulative amounts permeated in the order: receptor fluid (21.98 ± 1.19 μg/cm 2 ) > epidermis (15.40 ± 1.20 μg/cm 2 ) > dermis (9.52 ± 1.13 μg/cm 2 ). HS-GC-FID was successfully validated and the results point to DIL transdermal permeation and to the potential to develop pharmaceutical formulations for skin delivery to treat inflammation or infections.
Keyphrases
- gas chromatography
- essential oil
- mass spectrometry
- tandem mass spectrometry
- solid phase extraction
- high resolution mass spectrometry
- soft tissue
- gas chromatography mass spectrometry
- wound healing
- liquid chromatography
- anti inflammatory
- molecularly imprinted
- single cell
- oxidative stress
- cell therapy
- mesenchymal stem cells
- risk assessment
- quantum dots
- silver nanoparticles