In Vivo Detection of Age- and Disease-Related Increases in Neuroinflammation by 18F-GE180 TSPO MicroPET Imaging in Wild-Type and Alzheimer's Transgenic Mice.
Bin LiuKevin X LeMi-Ae ParkShuyan WangAnthony P BelangerShipra DubeyJeffrey L FrostPeter HoltonVladimir ReiserPaul A JonesWilliam TriggMarcelo F Di CarliCynthia A LemerePublished in: The Journal of neuroscience : the official journal of the Society for Neuroscience (2016)
Microglial activation, a player in Alzheimer's disease (AD) pathogenesis, is thought to reflect neuroinflammation. Using in vivo microPET imaging with a novel TSPO radioligand, (18)F-GE180, we detected significantly enhanced neuroinflammation during normal aging in WT mice and in response to AD-associated pathology in APP/PS1dE9 Tg mice, an AD mouse model. Increased uptake and specific binding of (18)F-GE180 in whole brain and hippocampus were confirmed by ex vivo PET and autoradiography. The binding specificity and stability of (18)F-GE180 was further confirmed by a cold tracer competition study and a metabolite study, respectively. Therefore, (18)F-GE180 PET imaging may be useful for longitudinal monitoring of neuroinflammation during AD progression and treatment and may also be useful for other neurodegenerative diseases.
Keyphrases
- pet imaging
- lipopolysaccharide induced
- lps induced
- wild type
- cerebral ischemia
- traumatic brain injury
- cognitive impairment
- mouse model
- positron emission tomography
- high resolution
- inflammatory response
- computed tomography
- cognitive decline
- type diabetes
- blood brain barrier
- high fat diet induced
- brain injury
- pet ct
- skeletal muscle
- subarachnoid hemorrhage
- dna binding
- photodynamic therapy
- combination therapy
- resting state
- quantum dots
- adipose tissue
- transcription factor
- fluorescence imaging
- smoking cessation
- real time pcr