Antitumour effects of a solid lipid nanoparticle loaded with gemcitabine and oxaliplatin on the viability, apoptosis, autophagy, and Hsp90 of ovarian cancer cells.
Ashwaq A Al-MutairiMayson H AlkhatibPublished in: Journal of microencapsulation (2022)
The present study aimed to explore the sensitising capability of the anticancer agents, gemcitabine (GEM) and oxaliplatin (OXA), encapsulated in a novel SLN (GEM:OXA-SLN) against the ovarian cancer cell lines. A novel SLN, prepared using hot homogenisation by mixing phosphatidylcholine, cholesterol, tween 80, and oleic acid, was characterised using Transmission Electron Microscope and zetasizer. The anticancer activities and the underlying molecular mechanisms of GEM:OXA-SLN were investigated. The average z -diameter of the homogeneous spherical GEM:OXA-SLN was (70.33 ± 0.70) nm with zeta potential (-7.69 ± 0.61) mV. GEM:OXA-SLN significantly inhibited the viability of ovarian cancer cells in a dose-dependent manner within 24 h. It also triggered the induction of autophagy cellular death, suppression of multidrug resistance efflux pump and inhibition of heat shock protein (Hsp90). The encapsulation of GEM and OXA in SLN improved the efficacy of the drugs and diminished the ovarian cancer cell's resistance.
Keyphrases
- acinetobacter baumannii
- heat shock protein
- klebsiella pneumoniae
- sentinel lymph node
- multidrug resistant
- drug resistant
- cell death
- endoplasmic reticulum stress
- heat shock
- oxidative stress
- pseudomonas aeruginosa
- escherichia coli
- early stage
- signaling pathway
- lymph node
- heat stress
- drug delivery
- neoadjuvant chemotherapy
- radiation therapy
- squamous cell carcinoma
- cystic fibrosis
- risk assessment
- high speed
- low density lipoprotein
- iron oxide