Syringoleosides A-H, Secoiridoids from Syringa dilatata Flowers and Their Inhibition of NO Production in LPS-Induced RAW 264.7 Cells.
Jung Eun GwagYeong-Geun LeeHyoung-Geun KimDong-Sung LeeDae Young LeeNam-In BaekPublished in: Journal of natural products (2020)
Repeated column chromatography of Syringa dilatata flowers, a native shrub to Korea, led to the isolation of eight new oleoside-type secoiridoids, syringoleosides A-H (1-8), as well as five known secoiridoids (9-13). The new chemical structures were identified through spectroscopic data analysis, as well as the application of chemical methods. Compounds 1, 2, 6, 7, 11, and 13 showed suppression effects on NO production in LPS-induced RAW 264.7 cells, with IC50 values ranging from 32.5 ± 9.8 to 65.7 ± 11.0 μM, and no visible toxicity. The content of the major secoiridoids in S. dilatata flowers, compounds 1, 4, 5, 8, 9, 12, and 13, were determined through HPLC analysis.
Keyphrases
- lps induced
- inflammatory response
- induced apoptosis
- data analysis
- cell cycle arrest
- mass spectrometry
- oxidative stress
- liquid chromatography
- endoplasmic reticulum stress
- high resolution
- molecular docking
- high performance liquid chromatography
- cell death
- signaling pathway
- solid phase extraction
- high speed
- cell proliferation
- pi k akt