LoopTag FRET Probe System for Multiplex qPCR Detection of Borrelia Species.

Henning HanschmannStefan RödigerToni KramerKatrin HanschmannMichael SteidleVolker Fingerle Carsten SchmidtWerner LehmannPeter Schierack

Published in: Life (Basel, Switzerland) (2021)

We established a novel multiplex probe real-time PCR system, designated LoopTag, that is simple, robust, and incorporates melting curve analysis for the detection and in the differentiation of European species belonging to the Borrelia burgdorferi s.l. complex.

Keyphrases

real time pcr
living cells
quantum dots
fluorescent probe
high resolution
single molecule
genetic diversity
energy transfer
high throughput
label free