Didymin improves UV irradiation resistance in C. elegans.
Lin ZhouLu WangJialing ZhangJiahe LiShuju BaiJunfeng MaXue-Qi FuPublished in: PeerJ (2019)
Didymin, a type of flavono-o-glycoside compound naturally present in citrus fruits, has been reported to be an effective anticancer agent. However, its effects on stress resistance are unclear. In this study, we treated Caenorhabditis elegans with didymin at several concentrations. We found that didymin reduced the effects of UV stressor on nematodes by decreasing reactive oxygen species levels and increasing superoxide dismutase (SOD) activity. Furthermore, we found that specific didymin-treated mutant nematodes daf-16(mu86) & daf-2(e1370), daf-16(mu86), akt-1(ok525), akt-2(ok393), and age-1(hx546) were susceptible to UV irradiation, whereas daf-2(e1371) was resistant to UV irradiation. In addition, we found that didymin not only promoted DAF-16 to transfer from cytoplasm to nucleus, but also increased both protein and mRNA expression levels of SOD-3 and HSP-16.2 after UV irradiation. Our results show that didymin affects UV irradiation resistance and it may act on daf-2 to regulate downstream genes through the insulin/IGF-1-like signaling pathway.
Keyphrases
- signaling pathway
- aqueous solution
- type diabetes
- reactive oxygen species
- cell proliferation
- pi k akt
- radiation induced
- epithelial mesenchymal transition
- gene expression
- binding protein
- amyotrophic lateral sclerosis
- nitric oxide
- heat shock protein
- newly diagnosed
- heat stress
- small molecule
- induced apoptosis
- endoplasmic reticulum stress
- protein protein
- bioinformatics analysis
- genome wide analysis