miR-30a-3p can inhibit the proliferation and promote the differentiation of chicken primary myoblasts.
Y LiP YuanS FanB ZhaiS LiH LiY ZhangWen-Ting LiG SunR HanY TianX LiuR JiangG LiX KangPublished in: British poultry science (2022)
1. Chicken muscle is an important factor in meat quality and its development is controlled by a complex regulatory network.2. The following study examined the expression of miR-30a-3p in Gushi chicken breast muscle tissue and found that it was differentially expressed at different embryonic stages, reaching a peak in the 14-day-old embryo (E14).3. The effect of miR-30a-3p on chicken primary myoblasts (CPMs) was explored. Results from both cell counting kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) showed that this can inhibit the proliferation of myoblasts, and through cell cycle experiments, the inhibition of myoblast proliferation was found, which may be due to G0/G1 arrest in the cell cycle.4. The effect of miR-30a-3p on the differentiation of myoblasts was studied. The results showed that miR-30a-3p can promote the expression of MYOD , myogenin ( MYOG ), and myosin heavy chain ( MYHC ) genes to promote the differentiation of myoblasts. Through MYHC protein immunofluorescence experiments, it was found that miR-30a-3p can effectively increase the area of myotubes.5. Finally, mRNA transcriptome data was analysed, which showed that miR-30a-3p has 51 potential target genes. Among them, forkhead box O3 ( FOXO3 ), ankyrin repeat domain 1 ( ANKRD1 ), and insulin-induced 1 ( INSIG1 ) genes were differentially expressed at different developmental stages and were enriched in Gene Ontology (GO) terms, such as cell differentiation and cellular developmental process. The data showed that tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma ( YWHAG ), BUB1 mitotic checkpoint serine/threonine kinase ( BUB1 ), and growth arrest and DNA damage-inducible 45 ( GADD45 ) genes were enriched in the cell cycle pathway.6. It can be speculated that miR-30a-3p plays roles through these genes in myoblast development. This research provides information for further improving knowledge of the chicken muscle development regulation network.
Keyphrases
- cell cycle
- genome wide
- cell proliferation
- genome wide identification
- binding protein
- transcription factor
- dna damage
- signaling pathway
- skeletal muscle
- bioinformatics analysis
- genome wide analysis
- dna methylation
- single cell
- type diabetes
- healthcare
- electronic health record
- gene expression
- cell therapy
- mesenchymal stem cells
- protein kinase
- copy number
- pi k akt
- tyrosine kinase
- high glucose
- big data
- endothelial cells
- glycemic control
- rna seq
- risk assessment
- machine learning
- small molecule
- protein protein
- diabetic rats