Optimizing Chitin Depolymerization by Lysozyme to Long-Chain Oligosaccharides.
Arnaud MasselinAntoine RousseauStéphanie PradeauLaure FortRodolphe GueretLaurine BuonSylvie ArmandSylvain CottazChoisnard LucSebastien FortPublished in: Marine drugs (2021)
Chitin oligosaccharides (COs) hold high promise as organic fertilizers in the ongoing agro-ecological transition. Short- and long-chain COs can contribute to the establishment of symbiotic associations between plants and microorganisms, facilitating the uptake of soil nutrients by host plants. Long-chain COs trigger plant innate immunity. A fine investigation of these different signaling pathways requires improving the access to high-purity COs. Here, we used the response surface methodology to optimize the production of COs by enzymatic hydrolysis of water-soluble chitin (WSC) with hen egg-white lysozyme. The influence of WSC concentration, its acetylation degree, and the reaction time course were modelled using a Box-Behnken design. Under optimized conditions, water-soluble COs up to the nonasaccharide were formed in 51% yield and purified to homogeneity. This straightforward approach opens new avenues to determine the complex roles of COs in plants.
Keyphrases
- water soluble
- signaling pathway
- air pollution
- transcription factor
- heavy metals
- machine learning
- hydrogen peroxide
- epithelial mesenchymal transition
- risk assessment
- resting state
- human health
- deep learning
- artificial intelligence
- oxidative stress
- pi k akt
- induced apoptosis
- binding protein
- cell wall
- plant growth
- electron transfer