Prevalence and Characterization of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Enterobacterales from Tunisian Seafood.
Mehdi SolaYosra ManiEstelle SarasAntoine DrapeauRaoudha GramiMahjoub AouniJean-Yves MadecMarisa HaenniWejdene MansourPublished in: Microorganisms (2022)
Aquaculture is a rapidly expanding sector in which it is important to monitor the occurrence of multi-drug resistant (MDR) bacteria. The presence of extended-spectrum β-lactamase (ESBL-) or carbapenemase-producing Enterobacterales is a commonly used indicator of the resistance burden in a given sector. In this study, 641 pieces of farmed fish (sea bream and sea bass), as well as 1075 Mediterranean clams, were analyzed. All ESBL- and carbapenemase-producing Enterobacterales collected were whole-genome sequenced. The proportion of ESBL-producing Enterobacterales was 1.4% in fish and 1.6% in clams, carried by Escherichia coli ( n = 23) and Klebsiella pneumoniae ( n = 4). The ESBL phenotype was exclusively due to the presence of bla CTX-M genes, the most frequent one being bla CTX-M-15 . The bla CTX-M-1 gene was also identified in six E. coli , among which four were carried by IncI1/pST3 plasmids, possibly betraying an animal origin. Carbapenemases were absent in fish but identified in two K. pneumoniae isolates from clams ( bla NDM-1 and bla OXA-48 ). Several sequence types (STs) identified were associated with human MDR clones such as E. coli ST131 and ST617, or K. pneumoniae ST307 and ST147. Our results might indicate that bacteria from hospital or farm effluents can reach the open sea and contaminate seafood and fish that are living or raised nearby. Therefore, monitoring the quality of water discharged to the sea and the presence of MDR bacteria in seafood is mandatory to ensure the quality of fishery products.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- drug resistant
- escherichia coli
- acinetobacter baumannii
- gram negative
- genome wide
- endothelial cells
- risk factors
- risk assessment
- wastewater treatment
- minimally invasive
- emergency department
- biofilm formation
- copy number
- genome wide identification
- amino acid
- transcription factor