Glass Nanopore Detection of Copper Ions in Single Cells Based on Click Chemistry.
Ping HuYong WangYing ZhangYongdong JinPublished in: Analytical chemistry (2022)
As a common redox metal ion pair in cells, copper ions (Cu<sup>2+</sup>/Cu<sup>+</sup>) often transform between oxidation (Cu<sup>2+</sup>) and reduction (Cu<sup>+</sup>) states. They play important roles in the redox process, so monitoring the change of intracellular copper ions helps understand the redox balance and events in cells. In this study, by self-assembling a thiolated ssDNA (with an alkyne end group) onto a gold-coated glass nanopore (G-nanopore) via the Au-S bond, an alkyne-end single-stranded DNA (ssDNA)-functionalized G-nanopore sensing platform (AG-nanopore) was developed to detect copper ions in cells. In the presence of Cu<sup>2+</sup> or Cu<sup>+</sup>, the introduction of another ssDNA with an azide group will be ligated with an alkyne group on the functionalized nanopore via a copper-catalyzed azide-alkyne 1,3-cycloaddition (CuAAC) click reaction and hence cause the change of the rectification behavior of the AG-nanopore. The rectification ratio variation of the AG-nanopore had a good response to the intracellular copper ion concentration, and the sensing platform was further applied to the study of the relationship between intracellular oxidative stress and the value of Cu<sup>2+</sup>/Cu<sup>+</sup>.
Keyphrases
- single molecule
- induced apoptosis
- aqueous solution
- quantum dots
- oxidative stress
- cell cycle arrest
- solid state
- metal organic framework
- endoplasmic reticulum stress
- signaling pathway
- dna damage
- cell death
- sensitive detection
- nitric oxide
- oxide nanoparticles
- reactive oxygen species
- highly efficient
- cell free
- circulating tumor
- circulating tumor cells
- diabetic rats
- heat shock protein
- nucleic acid