Mitochondria reorganization upon proliferation arrest predicts individual yeast cell fate.
Damien LaporteLaëtitia GoulemeLaure JimenezInes KhemiriIsabelle SagotPublished in: eLife (2018)
Most cells spend the majority of their life in a non-proliferating state. When proliferation cessation is irreversible, cells are senescent. By contrast, if the arrest is only temporary, cells are defined as quiescent. These cellular states are hardly distinguishable without triggering proliferation resumption, hampering thus the study of quiescent cells properties. Here we show that quiescent and senescent yeast cells are recognizable based on their mitochondrial network morphology. Indeed, while quiescent yeast cells display numerous small vesicular mitochondria, senescent cells exhibit few globular mitochondria. This allowed us to reconsider at the individual-cell level, properties previously attributed to quiescent cells using population-based approaches. We demonstrate that cell's propensity to enter quiescence is not influenced by replicative age, volume or density. Overall, our findings reveal that quiescent cells are not all identical but that their ability to survive is significantly improved when they exhibit the specific reorganization of several cellular machineries.
Keyphrases
- induced apoptosis
- cell cycle arrest
- signaling pathway
- cell death
- endoplasmic reticulum stress
- oxidative stress
- stem cells
- magnetic resonance
- gene expression
- magnetic resonance imaging
- dna methylation
- mesenchymal stem cells
- single cell
- cell therapy
- pi k akt
- reactive oxygen species
- endoplasmic reticulum
- genome wide
- neural stem cells