An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer.

Kazuo ImaiNorihito TarumotoLucky Ronald RuntuweneJun SakaiKyoko HayashidaYuki EshitaRyuichiro MaedaJosef TudaHideaki OhnoTakashi MurakamiShigefumi MaesakiYutaka SuzukiJunya YamagishiTakuya Maeda

Published in: Malaria journal (2018)

An innovative method combining LAMP and MinION will enable simple, rapid, and high-sensitivity detection of the C580Y mutation of P. falciparum, even in resource-limited situations in developing countries.

Keyphrases

loop mediated isothermal amplification
plasmodium falciparum
sensitive detection
single molecule
real time pcr
solid state