Oligodendrocyte-derived extracellular vesicles as antigen-specific therapy for autoimmune neuroinflammation in mice.
Giacomo CasellaJavad RasouliAlexandra BoehmWei-Feng ZhangDan XiaoLarissa Lumi Watanabe IshikawaRodolfo ThomeXing LiDaniel HwangPatrizia PorazziSudheer Kumar MoluguHsin-Yao TangGuang-Xian ZhangBogoljub CiricAbdolmohamad RostamiPublished in: Science translational medicine (2021)
Autoimmune diseases such as multiple sclerosis (MS) develop because of failed peripheral immune tolerance for a specific self-antigen (Ag). Numerous approaches for Ag-specific suppression of autoimmune neuroinflammation have been proven effective in experimental autoimmune encephalomyelitis (EAE), an animal model of MS. One such approach is intravenous tolerance induction by injecting a myelin Ag used for triggering EAE. However, the translation of this and similar experimental strategies into therapy for MS has been hampered by uncertainty regarding relevant myelin Ags in MS patients. To address this issue, we developed a therapeutic strategy that relies on oligodendrocyte (Ol)-derived extracellular vesicles (Ol-EVs), which naturally contain multiple myelin Ags. Intravenous Ol-EV injection reduced disease pathophysiology in a myelin Ag-dependent manner, both prophylactically and therapeutically, in several EAE models. The treatment was safe and restored immune tolerance by inducing immunosuppressive monocytes and apoptosis of autoreactive CD4+ T cells. Furthermore, we showed that human Ols also released EVs containing most relevant myelin Ags, providing a basis for their use in MS therapy. These findings introduce an approach for suppressing central nervous system (CNS) autoimmunity in a myelin Ag-specific manner, without the need to identify the target Ag.
Keyphrases
- multiple sclerosis
- white matter
- quantum dots
- mass spectrometry
- highly efficient
- ms ms
- visible light
- end stage renal disease
- traumatic brain injury
- chronic kidney disease
- oxidative stress
- high dose
- lipopolysaccharide induced
- endothelial cells
- cognitive impairment
- metabolic syndrome
- type diabetes
- lps induced
- blood brain barrier
- cell proliferation
- prognostic factors
- dendritic cells
- patient reported outcomes
- peripheral blood
- combination therapy
- mesenchymal stem cells
- subarachnoid hemorrhage
- cell therapy