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BARCOSEL: a tool for selecting an optimal barcode set for high-throughput sequencing.

Panu SomervuoPatrik KoskinenPeng MeiLiisa HolmPetri AuvinenLars Paulin
Published in: BMC bioinformatics (2018)
Increasing capacity of sequencing platforms raises the challenge of mixing barcodes. Our method allows the user to select a given number of barcodes among the larger existing barcode set so that both sequencing errors are tolerated and the nucleotide balance is optimized. The tool is easy to access via web browser.
Keyphrases
  • high throughput sequencing
  • single cell
  • patient safety
  • emergency department
  • quality improvement