Oxidized Alginate Dopamine Conjugate: A Study to Gain Insight into Cell/Particle Interactions.
Adriana TrapaniFilomena Faustina Rina CorboErika StefànoLoredana CapobiancoAntonella MuscellaSanto MarsiglianteAntonio CricentiMarco LuceDavid BecerrilStefano BellucciPublished in: Journal of functional biomaterials (2022)
Background : We had previously synthetized a macromolecular prodrug consisting of oxidized Alginate and dopamine (AlgOx-Da) for a potential application in Parkinson disease (PD). Methods : In the present work, we aimed at gaining an insight into the interactions occurring between AlgOx-Da and SH-SY5Y neuronal cell lines in view of further studies oriented towards PD treatment. With the scope of ascertaining changes in the external and internal structure of the cells, multiple methodologies were adopted. Firstly, fluorescently labeled AlgOx-Da conjugate was synthetized in the presence of fluorescein 5(6)-isothiocyanate (FITC), providing FITC-AlgOx-Da, which did not alter SH-SY5Y cell viability according to the sulforhodamine B test. Furthermore, the uptake of FITC-AlgOx-Da by the SH-SY5Y cells was studied using scanning near-field optical microscopy and assessments of cell morphology over time were carried out using atomic force microscopy. Results : Notably, the AFM methodology confirmed that no relevant damage occurred to the neuronal cells. Regarding the effects of DA on the intracellular reactive oxygen species (ROS) production, AlgOx-Da reduced them in comparison to free DA, while AlgOx did almost not influence ROS production. Conclusions : these findings seem promising for designing in vivo studies aiming at administering Oxidized Alginate Dopamine Conjugate for PD treatment.
Keyphrases
- reactive oxygen species
- induced apoptosis
- atomic force microscopy
- parkinson disease
- cell cycle arrest
- high speed
- cell death
- high resolution
- cancer therapy
- oxidative stress
- dna damage
- endoplasmic reticulum stress
- cell therapy
- risk assessment
- metabolic syndrome
- low density lipoprotein
- computed tomography
- bone marrow
- pet ct
- label free