Oleic Acid Coated Silver Nanoparticles Showed Better in Vitro Amoebicidal Effects against Naegleria fowleri than Amphotericin B.
Kavitha RajendranAyaz AnwarNaveed Ahmed KhanZara AslamMuhammad Raza ShahRuqaiyyah SiddiquiPublished in: ACS chemical neuroscience (2019)
Naegleria fowleri (N. fowleri) causes primary amoebic meningoencephalitis (PAM) which almost always results in death. N. fowleri is also known as "brain-eating amoeba" due to its literal infestation of the brain leading to an inflammatory response in the brain tissues. Currently, there is no single drug that is available to treat PAM, and most treatments are combinations of antifungal, anticancer, and anti-inflammatory drugs. Recently nanotechnology has gained attention in chemotherapeutic research converging on drug delivery, while oleic acid (OA) has shown positive effects on the human immune system and inflammatory processes. In continuation of our recent research in which we reported the effects of oleic acid conjugated with silver nanoparticles (OA-AgNPs) against free-living amoeba Acanthamoeba castellanii, in this report, we show their antiamoebic effects against N. fowleri. OA alone and its nanoconjugates were tested against the amoeba by using amoebicidal and host cell cytopathogenicity assays. Trypan blue exclusion assay was used to determine cell viability. The results revealed that OA-AgNPs exhibited significantly enhanced antiamoebic effects (P < 0.05) against N. fowleri as compared to OA alone. Evidently, lactate dehydrogenase release shows reduced N. fowleri-mediated host cell cytotoxicity. Based on our study, we anticipate that further studies on OA-AgNPs could potentially provide an alternative treatment of PAM.
Keyphrases
- silver nanoparticles
- knee osteoarthritis
- inflammatory response
- single cell
- drug delivery
- white matter
- endothelial cells
- resting state
- physical activity
- high throughput
- photodynamic therapy
- gene expression
- multiple sclerosis
- oxidative stress
- candida albicans
- cancer therapy
- bone marrow
- drug release
- weight loss
- brain injury
- subarachnoid hemorrhage