HER2 aptamer-conjugated iron oxide nanoparticles with PDMAEMA-b-PMPC coating for breast cancer cell identification.
Cyro von Zuben de Valega NegrãoNatália Np CerizeAmauri da Silva Justo-JuniorRaquel Bester LiszbinskiGiovanna Pastore MeneguettiLarissa AraujoSilvana A RoccoKaliandra de Almeida GonçalvesDaniel R CornejoPatrícia LeoCaio PerecinDouglas AdamoskiSandra Martha Gomes DiasPublished in: Nanomedicine (London, England) (2024)
Aim: To synthesize HER2 aptamer-conjugated iron oxide nanoparticles with a coating of poly(2-(dimethylamino) ethyl methacrylate)-poly(2-methacryloyloxyethylphosphorylcholine) block copolymer (IONPPPs). Methods: Characterization covered molecular structure, chemical composition, thermal stability, magnetic characteristics, aptamer interaction, crystalline nature and microscopic features. Subsequent investigations focused on IONPPPs for in vitro cancer cell identification. Results: Results demonstrated high biocompatibility of the diblock copolymer with no significant toxicity up to 150 μg/ml. The facile coating process yielded the IONPP complex, featuring a 13.27 nm metal core and a 3.10 nm polymer coating. Functionalized with a HER2-targeting DNA aptamer, IONPPP enhanced recognition in HER2-amplified SKBR3 cells via magnetization separation. Conclusion: These findings underscore IONPPP's potential in cancer research and clinical applications, showcasing diagnostic efficacy and HER2 protein targeting in a proof-of-concept approach.
Keyphrases
- iron oxide nanoparticles
- gold nanoparticles
- sensitive detection
- photodynamic therapy
- magnetic nanoparticles
- quantum dots
- label free
- induced apoptosis
- cancer therapy
- single molecule
- molecularly imprinted
- oxidative stress
- drug release
- cell cycle arrest
- ionic liquid
- squamous cell carcinoma
- circulating tumor
- room temperature
- signaling pathway
- cell free
- cell death
- endoplasmic reticulum stress
- binding protein
- human health
- climate change
- amino acid
- protein protein