Development of a multiplex TaqMan qPCR targeting unique genomic regions for the specific and sensitive detection of Pectobacterium species and P. parmentieri.

Dario ArizalaShefali DobhalBrooke BablerAlex B CrockfordRenee A RiouxAnne M AlvarezMohammad Arif

Published in: Journal of applied microbiology (2022)

The developed assay can be adopted for multiple purposes such as seed certification programmes, surveillance, biosecurity, microbial forensics, quarantine, border protection, inspections and epidemiology.

Keyphrases

sensitive detection
real time pcr
high throughput
quantum dots
public health
microbial community
loop mediated isothermal amplification
risk factors
cancer therapy
gene expression
single cell
dna methylation