Calcium Imaging and Electrophysiology of hippocampal Activity under Anesthesia and natural Sleep in Mice.
Andrey FormozovMattia ChiniAlexander DieterWei YangJastyn A PöpplauIleana L Hanganu-OpatzJ Simon WiegertPublished in: Scientific data (2022)
The acute effects of anesthesia and their underlying mechanisms are still not fully understood. Thus, comprehensive analysis and efficient generalization require their description in various brain regions. Here we describe a large-scale, annotated collection of 2-photon calcium imaging data and multi-electrode, extracellular electrophysiological recordings in CA1 of the murine hippocampus under three distinct anesthetics (Isoflurane, Ketamine/Xylazine and Medetomidine/Midazolam/Fentanyl), during natural sleep, and wakefulness. We cover several aspects of data quality standardization and provide a set of tools for autonomous validation, along with analysis workflows for reuse and data exploration. The datasets described here capture various aspects of neural activity in hundreds of pyramidal cells at single cell resolution. In addition to relevance for basic biological research, the dataset may find utility in computational neuroscience as a benchmark for models of anesthesia and sleep.
Keyphrases
- electronic health record
- high resolution
- single cell
- physical activity
- big data
- sleep quality
- rna seq
- liver failure
- oxidative stress
- type diabetes
- data analysis
- white matter
- photodynamic therapy
- insulin resistance
- respiratory failure
- drug induced
- adipose tissue
- metabolic syndrome
- pain management
- signaling pathway
- acute respiratory distress syndrome
- deep learning
- intensive care unit
- mass spectrometry
- living cells
- fluorescence imaging
- chronic pain
- aortic dissection
- quality improvement
- temporal lobe epilepsy
- mechanical ventilation