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Scalarane Sesterterpenoids with Antibacterial and Anti-Proliferative Activities from the Mushroom Neonothopanus   nambi .

Awat WisetsaiRatsami LekphromSureeporn Bua-ArtThanapat SuebrasriSophon BoonlueSarawut TontaphaVittaya AmornkitbamrungThanaset SenawongFlorian T Schevenels
Published in: Molecules (Basel, Switzerland) (2021)
Seven undescribed scalarane sesterterpenoids, nambiscalaranes B-H ( 1 - 7 ), together with two known compounds, nambiscalarane ( 8 ) and aurisin A ( 9 ) were isolated from the cultured mycelium of the luminescent mushroom Neonothopanus nambi . Their structures were elucidated by thorough analysis of their 1D and 2D NMR spectroscopic data. The absolute configurations of 1 - 8 were determined by electronic circular dichroism (ECD) calculations and optical rotation measurements. The isolated sesterterpenoids were evaluated against A549, HT29, HeLa, and HCT-116 cancer cell lines, and against five bacterial strains. Compounds 3 , 5 , and 7 showed strong cytotoxicity against HCT-116 cell line, with IC 50 values ranging from 13.41 to 16.53 µM, and showed no cytotoxicity towards Vero cells. Moreover, compound 8 inhibited the growth of Bacillus subtilis with a MIC value of 8 µg/mL, which was equivalent to the MIC value of the standard kanamycin.
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