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Bioprospecting of Helichrysum Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against Botrytis cinerea .

Neliswa A MatroseZinash A BelayKenechukwu ObikezeLucky MokwenaOluwafemi James Caleb
Published in: Plants (Basel, Switzerland) (2022)
Variation in plant species and extraction solvents play a crucial role in the recovery of their bioactive compounds and antifungal efficacy. Thus, in this study, a comparative investigation was carried out using extraction solvents: 70% acetone and 95% ethanol to obtain crude aqueous extracts from Helichrysum odoratissimum and H. patulum . Crude aqueous extracts were screened using gas chromatography-mass spectrometry (GC-MS), to gain insight into their chemical composition. Phytochemical properties (total polyphenols (TP) and radical scavenging capacity via 2,2-diphenyl-1-picrylhydrazyl (DPPH)), and antifungal activity against Botrytis cinerea of the crude extracts were evaluated. Fungicide (Rovral ® WP) and extraction solvents were used as controls. Variation in Helichrysum spp. and extraction solvent had influence on the chemical composition, phytochemicals, and antifungal activities. Metabolites such as γ-terpinene (≈0.1%), α-amorphene (≈0.6%) α-gurjunene (≈1.4%), β-selinene (2.2-3.2%), γ-gurjunene (≈3.3%), and methyl cinnamate (≈20%) were detected only in extracts of H. patulum . Crude extract of H. odoratissimum using 70% acetone had the highest TP (19.3 ± 0.76 g GA 100 g -1 ), and DPPH capacity (13,251.5 ± 700.55 µmol Trolox g -1 ) compared to H. patulum ( p ≤ 0.05). Ethanolic extracts of H. patulum showed highest antifungal efficacy (≈65%) against B. cinerea ( p ≤ 0.05) compared to other crude extracts. This study showed that Helichrysum spp. differ in their potential as a source for bioactive compounds and antifungal treatments/formulations.
Keyphrases
  • candida albicans
  • ionic liquid
  • gas chromatography mass spectrometry
  • pet ct
  • ms ms
  • risk assessment
  • gas chromatography