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Genetics and Gene Editing Methods in Xenopus laevis and Xenopus tropicalis .

Matthew J GuilleRobert Grainger
Published in: Cold Spring Harbor protocols (2022)
Our understanding of biological systems has for many years been heavily influenced by experimental approaches that exploit genetic methods. These include gain-of-function experiments that overexpress transgenes or ectopically express injected RNA and loss-of-function experiments that knock out genes or knock down RNAs. Here, we review how these methods have been applied in Xenopus frogs and introduce a variety of protocols for genetic manipulation of Xenopus laevis and Xenopus tropicalis .
Keyphrases
  • cell free
  • genome wide
  • copy number
  • transcription factor
  • genome wide identification